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Characterization of Transient Cultures of Clostridium acetobutylicum
Author(s) -
Srivastava Ashok K.,
Volesky Bohumil
Publication year - 1990
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00006a002
Subject(s) - clostridium acetobutylicum , acidogenesis , clostridium , flux balance analysis , transient (computer programming) , bottleneck , biochemistry , biological system , chemistry , biochemical engineering , biology , computer science , bacteria , engineering , butanol , ethanol , genetics , ecology , methane , embedded system , anaerobic digestion , operating system
Abstract The inability of unstructured mathematical models to acknowledge complex intracellular physiological changes limits their applicability for dynamic culture growth conditions. By considering RNA as a bottleneck growth‐limiting compound, a structured model was developed to describe the transient and steady‐state growth of Clostridium acetobutylicum. The linear relationship between the on‐line NADH fluorescence and the sugar utilization specific rates was incorporated in the mathematical model to facilitate in situ assessment of the culture metabolic activity. The improved performance of the proposed structured model was demonstrated. It simulated the transient culture metabolism better than the unstructured Monod model. However, it still failed to simulate the metabolic switchover between acidogenic and solventogenic Clostridium culture behavior.