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Cybernetic Modeling and Regulation of Metabolic Pathways in Multiple Steady States of Hybridoma Cells
Author(s) -
Guardia Maria Jesus,
Gambhir Anshu,
Europa Anna F.,
Ramkrishna Doraiswami,
Hu WeiShou
Publication year - 2000
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp000069a
Subject(s) - steady state (chemistry) , cybernetics , substrate (aquarium) , biological system , glutamine , metabolic regulation , metabolism , mode (computer interface) , state (computer science) , biology , chemistry , computer science , biochemistry , ecology , artificial intelligence , algorithm , amino acid , operating system
Abstract Hybridoma cells utilize a pair of complementary and partially substitutable substrates, glucose and glutamine, for growth. It has been shown that cellular metabolism shifts under different culture conditions. When those cultures at different metabolic states are switched to a continuous mode, they reach different steady states under the same operating conditions. A cybernetic model was constructed to describe the complementary and partial substitutable nature of substrate utilization. The model successfully predicted the metabolic shift and multiple steady‐state behavior. The results are consistent with the experimental observation that the history of the culture affects the resulting steady state.