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Medium Optimization of Transformed Root Cultures of Stizolobium hassjoo Producing L −DOPA with Response Surface Methodology
Author(s) -
Sung LinShiang,
Huang ShihYow
Publication year - 2000
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp000062t
Subject(s) - central composite design , response surface methodology , fractional factorial design , chemistry , dry weight , factorial experiment , dilution , food science , horticulture , chromatography , botany , mathematics , biology , physics , statistics , thermodynamics
Medium optimization of B5 medium for hairy root cultures producing secondary metabolites was studied through statistical experimental design. Transformed root cultures of Stizolobium hassjoo producing L −DOPA were used as a model system. The serial dilution experiments facilitated logical choice of the upper and lower bounds on executing 2 11 −6 fractional factorial design. Steepest ascent method as well as central composite design were sequentially employed to optimize the media of shake flask cultures. The modified B5 media of GM, PM, and GPM were obtained, indicating the optimum medium compositions for enhancing hairy root dry weight, L −DOPA content in hairy roots, and L −DOPA production, respectively. When cultivating S. hassjoo hairy roots in GM, PM and GPM for 16 days, the dry wt of hairy roots, L −DOPA content, and L −DOPA production obtained were ca. 530 mg per flask (10.6 g/L), 10.8% dry wt, and 806 mg/L, which were 1.8‐, 2‐, and 2.8‐fold of basal B5 medium control runs, respectively.