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Practical Considerations in the Measurement of Culture Fluorescence
Author(s) -
Coppella Steven J.,
Rao Govind
Publication year - 1990
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00005a013
Subject(s) - aeration , fluorescence , bioreactor , in situ , flow (mathematics) , volumetric flow rate , environmental science , analytical chemistry (journal) , chromatography , biological system , chemistry , optics , biology , mechanics , physics , organic chemistry
We have examined practical considerations associated with the use of a commercially available fluorescence probe for in situ measurements in bioreactors. The optical path length of the measurement was first determined and a flow cell subsequently designed. The environment (agitation/aeration rates) of the probe was found to have a significant influence on the measurement. These effects were eliminated by placing the probe in a recycle loop using the flow cell. Fluorescence measurement in the recycle loop was verified to be representative of the cell sample and to not affect cell metabolism.