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Fluidized Bed Adsorption for Whole Broth Extraction
Author(s) -
Gailliot F. P.,
Gleason C.,
Wilson J. J.,
Zwarick J.
Publication year - 1990
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00005a009
Subject(s) - fluidized bed , adsorption , extraction (chemistry) , chromatography , pilot plant , fermentation , pulp and paper industry , yield (engineering) , downstream processing , process engineering , solvent , chemistry , environmental science , waste management , materials science , organic chemistry , engineering , metallurgy
Fluidized bed adsorption using a high‐density synthetic resin has proven to be an invaluable technique for separating novel compounds from unfiltered fermentation broths during the very early stages of fermentation development, where product concentrations are typically in the parts per million range. Previous initial downstream processing strategies consisted of cell separation from whole broth or direct extraction with water‐immiscible solvents, both of which resulted in lengthy time cycles, conflicts with existing operations requiring the use of high‐cost centrifugal separators, and environmental/solvent recovery concerns. Laboratory and subsequent pilot plant process development work along with concomitant improvements in yield, quality, and time cycles are presented for one of several fluidized bed processes piloted in Merck's Natural Product Isolation facility.