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Growth and Fermentation Model for Alginate‐Entrapped Saccharomyces cerevisiae
Author(s) -
Hannoun Betty J. M.,
Stephanopoulos Gregory
Publication year - 1990
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00005a006
Subject(s) - bioreactor , fermentation , saccharomyces cerevisiae , diffusion , steady state (chemistry) , suspension (topology) , chemistry , chromatography , chemical engineering , yeast , biological system , biochemistry , biophysics , biology , thermodynamics , organic chemistry , mathematics , engineering , physics , homotopy , pure mathematics
Abstract A model of growth and fermentation for alginate‐entrapped Saccharomyces cerevisiae is presented. The model is based on Peringer's rate equations (Peringer, P.; et al. Biotechnol. Bioeng. Symp. 1973 , 4, 27–42), developed for glucose‐repressed cultures of suspended cells at varying dissolved oxygen concentrations, and was fitted to estimates of growth and fermentation rates for immobilized cells obtained through a combination of experimental measurements and reaction‐diffusion analysis. Immobilized cell fermentation data with alginate beads in suspension compared well with simulations of reaction and diffusion in a similar system incorporating the proposed growth‐fermentation model. In view of these findings, the model was further applied to the simulation of transient and steady‐state operation of a packed‐bed immobilized cell biore‐actor. Results about the effect of operating parameters on bioreactor performance are presented, and implications regarding system design and operation for optimal overall performance are discussed.