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Comparative Studies of Glucose Catabolism by Escherichia coli Grown in a Complex Medium under Aerobic and Anaerobic Conditions
Author(s) -
DiazRicci Juan C.,
Hitzmann Bernd,
Rinas Ursula,
Bailey James E.
Publication year - 1990
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00005a003
Subject(s) - anaerobic exercise , catabolite repression , catabolism , biochemistry , escherichia coli , chemistry , growth medium , intracellular , metabolism , biology , bacteria , physiology , genetics , mutant , gene
Escherichia coli HB101 was grown in complex medium under anaerobic and aerobic conditions. Cells prepared under these two different conditions were characterized by two‐dimensional protein gel electrophoresis, by NMR measurements under identical (anaerobic) conditions, and by measuring the kinetics of glucose uptake and catabolite end‐product appearance in the medium under identical anaerobic conditions. Specific rates of glucose uptake and end‐product formation were significantly greater for the anaerobically grown cells, which also exhibited lower intracellular concentrations of sugar phosphates, nucleoside di‐and triphosphates, UDPG, and NAD(H). Two‐dimensional gel electrophoretic analyses reveal changes in the intracellular levels of proteins involved in pyruvate catabolism that have been observed previously for E. coli grown in minimal medium under aerobic and anaerobic conditions. Enzymes involved in the TCA cycle were not detected in cells grown aerobically or anaerobically in complex medium.