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N ‐Laurylbiotinamide as Affinity Surfactant
Author(s) -
Coughlin Robert W.,
Baclaski Jeffrey B.
Publication year - 1990
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00004a012
Subject(s) - pulmonary surfactant , avidin , biotin , chemistry , aqueous solution , chromatography , phase (matter) , micelle , aqueous two phase system , organic chemistry , biochemistry
N ‐laurylbiotinamide (NLB), which retains strong affinity for the protein avidin, was synthesized from biotin and N ‐laurylamine via the biotin ester of N ‐hydroxysuccinimide and characterized by NMR. When the synthesized NLB was used as a cosurfactant with AOT to form a reverse micellar system in isooctane, it was found to extend the pH range over which avidin can be transferred from a continuous aqueous solution to the reverse‐micellar phase. This behavior is similar to that already reported for a different affinity surfactant, n ‐octyl β‐D‐glucopyranoside.