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Biocatalytic Transformation of [(2‐Hydroxyethyl)thio]acetic Acid and Thiodiglycolic Acid from Thiodiglycol by Alcaligenes xylosoxydans ssp. xylosoxydans (SH91)
Author(s) -
Lee Tsushun,
Chan ShuHua,
Weigand William A.,
Bentley William E.
Publication year - 2000
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp000044b
Subject(s) - chemistry , acetic acid , hydrolysis , sulfur mustard , yield (engineering) , batch reactor , chromatography , organic chemistry , biochemistry , catalysis , materials science , toxicity , metallurgy
A Gram‐negative bacterium, Alcaligenes xylos oxyd ans ssp . xylos oxyd ans (SH91), consumed thiodiglycol (TDG), the nontoxic hydrolysis product of sulfur mustard, as a primary carbon source and transformed TDG to commercially relevant chemical precursors, [(2‐hydroxyethyl)thio]acetic acid (HETA) and thiodiglycolic acid (TDGA). Aerobic fed batch and repeated batch experiments were run to compare the molar yields of HETA and TDGA that result under different operating policies. In repeated batch experiments, 35% of the TDG was converted to HETA. Under the conventional batch process and a repeated fed batch process, the HETA yields were reduced (21% and 18%, respectively), while the yield of TDGA was increased (47% and 31%,respectively). This work demonstrated that cell growth associated biocatalytic transformations were manipulated to achieve a desired byproducts profile through an understanding of the specific reaction and cell growth kinetics and by altering the reaction operating policy accordingly.