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Production of Monoclonal Antibodies by Hybridoma Cells in a Flat Sheet Membrane Bioreactor
Author(s) -
Hagedorn J.,
Kargi F.
Publication year - 1990
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00003a011
Subject(s) - monoclonal antibody , membrane , bioreactor , diffusion , chemistry , membrane bioreactor , antibody , biophysics , biology , biochemistry , immunology , organic chemistry , thermodynamics , physics
Abstract The purpose of this study was to investigate hybridoma growth and monoclonal antibody formation in a flat sheet membrane bioreactor. The effects of several different molecular weight cutoff membranes on growth and antibody formation were investigated. Nutrient and toxic product diffusion through the membranes were quantified, and the kinetic and physical constants of the system were determined.