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Recovery of Clostridium thermosulfurogenes Produced β‐Amylase by (Hydroxypropyl)methylcellulose Partition
Author(s) -
Miranda Everson A.,
Berglund Kris A.
Publication year - 1990
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00003a010
Subject(s) - chemistry , ammonium sulfate , aqueous solution , chromatography , fermentation , polymer , centrifugation , ethylene glycol , amylase , ammonium , partition coefficient , enzyme , organic chemistry
A procedure for recovering Clostridium thermosulfurogenes produced β‐amylase from fermentation broth by partition was developed. The partition was achieved by addition of ammonium sulfate to an aqueous solution of the enzyme with (hydroxypropyl)methylcellulose. The β‐amylase‐containing pellet formed upon centrifugation could be redissolved and the polymer recovered by a second salt addition. The process was not dependent on polymer/enzyme solution pH, but it was affected by temperature, polymer nominal molecular weight and loading, and fermentation carbon source. Unlike more traditional aqueous‐phase partitions, such as poly(ethylene glycol)/dextran, the current approach appeared to be biospecific.