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Multilayer Binding of Proteins to Polymer Chains Grafted onto Porous Hollow‐Fiber Membranes Containing Different Anion‐Exchange Groups
Author(s) -
Koguma Ichiro,
Sugita Kazuyuki,
Saito Kyoichi,
Sugo Takanobu
Publication year - 2000
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp000036v
Subject(s) - membrane , polymer , polymer chemistry , monomer , chemistry , fiber , epoxy , ion exchange , permeation , polymerization , protein adsorption , adsorption , surface modification , side chain , bovine serum albumin , chemical engineering , ion , chromatography , organic chemistry , biochemistry , engineering
Various anion‐exchange groups were introduced into the polymer chains grafted onto a porous hollow‐fiber membrane for protein recovery by radiation‐induced graft polymerization and subsequent functionalization of a monomer containing an epoxy group. The graft chains extended from the pore surface toward the pore interior, resulting in the multilayer binding of proteins to the graft chains. Combinations of three anion‐exchange groups, namely, amino (AM), ethylamino (EA), and diethylamino (DEA) groups, and three proteins, namely, β‐lactoglobulin, bovine serum albumin, and urease, were examined to evaluate the degree of multilayer binding of protein to the graft chains in the permeation mode. Multilayer binding was observed for hollow‐fiber membranes containing EA and DEA groups, with conversions of epoxy groups to EA or DEA groups of higher than 80%. The amount of adsorbed protein remained constant irrespective of the conversion for the hollow‐fiber membrane containing an AM group. The dependence of the flux on the conversion was consistent with that of the degree of multilayer binding to the graft chains.