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Effect of Inclusion Body Production on Culture Turbidity and Cell Dry Weight in Growing Bacterial Cultures
Author(s) -
Hwang Soon Ook,
Feldberg Ross S.
Publication year - 1990
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00001a007
Subject(s) - turbidity , dry weight , yield (engineering) , chemistry , cell , food science , biology , biochemistry , botany , ecology , materials science , metallurgy
An approach to the optimization of product yield in an inducible inclusion body‐producing system is presented. Following induction by indoleacrylic acid (IAA) of a trpLEHIVgp41 fusion protein, we found a large increase in culture turbidity and single‐cell dry weight. After an initial transition phase, new and constant values for specific growth rate, single‐cell light turbidity, and single cell dry weight were achieved, allowing for the determination of optimal conditions for product formation.