Premium
New Polymers Forming Aqueous Two‐Phase Polymer Systems
Author(s) -
Pietruszka N.,
Galaev I. Yu.,
Kumar A.,
Brzozowski Z. K.,
Mattiasson B.
Publication year - 2000
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp000013g
Subject(s) - copolymer , acrylamide , starch , aqueous solution , polymer , ethylene glycol , hydrolysis , chemistry , polymer chemistry , aqueous two phase system , phase (matter) , nuclear chemistry , organic chemistry
A new type of polymer, starch‐modified by acrylamide, has been developed for application in aqueous two‐phase systems (ATPS) for protein separation. Partial hydrolysis and acrylamide modification of starch to different degrees make it suitable for forming ATPS with poly(ethylene glycol) in a moderate concentration range. The potential of the polymer to form ATPS with the thermoprecipitating copolymer of 1‐vinylimidazole with N ‐vinylcaprolactam (poly‐VI/VCL) has been evaluated. The thermoprecipitation properties of poly‐VI/VCL and Cu(II)‐loaded poly‐VI/VCL have been studied for application in metal affinity partitioning. The formation of ATPS with Cu(II)‐loaded thermoprecipitating copolymer was critically achieved for poly‐VI/VCL (10/90) copolymer in under‐loaded metal concentrations. With the Cu(II)‐loaded copolymer, poly‐VI/VCL in the top phase and modified starch in the bottom phase, the ATPS formed was used for the purification of α‐amylase inhibitor from wheat meal. The protein partitioned in the top phase and phase‐separated polymer‐protein complex could be precipitated by salt. The protein inhibitor was recovered with a yield of 75%.