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Enzymatically and Combinatorially Generated Array‐Based Polyphenol Metal Ion Sensor
Author(s) -
Wu Xiaoqiu,
Kim Jungbae,
Dordick Jonathan S.
Publication year - 2000
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0000104
Subject(s) - monomer , metal ions in aqueous solution , polymerization , copolymer , polymer , metal , polyphenol , fluorescence , chemistry , materials science , polymer chemistry , organic chemistry , physics , quantum mechanics , antioxidant
Phenolic polymers were synthesized via soybean hull peroxidase catalysis and used as metal‐based sensor components in a polymer array. A sensor array for Fe 3+ , Cu 2+ , Co 2+ , and Ni 2+ has been developed consisting of 15 phenolic homopolymers and copolymers generated from five phenolic monomers by peroxidase‐catalyzed oxidative polymerization. Sensing was based on the change of intrinsic polyphenol fluorescence upon addition of a metal ion or a metal ion mixture to an aqueous suspension of a polyphenol. Importantly, the fluorescence response of copolymers differed, in some cases dramatically, from the constituent homopolymers and was dependent upon the relative ratio of monomers that comprise the polymer. This finding suggests that an extremely broad range of sensor arrays can be generated from a limited number of phenolic monomers. Using a statistical analysis, histograms constructed for the four different metal ions yielded unique fingerprints of the array response and can be used to identify specific metal ions.