Optical Control of Peroxisomal Trafficking | Zendy

Jessica I. Spiltoir | Zendy; Devin Strickland | Zendy; Michael Glotzer | Zendy; Chandra L. Tucker | Zendy

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Optical Control of Peroxisomal Trafficking

Author(s) -

Jessica I. Spiltoir,

Devin Strickland,

Michael Glotzer,

Chandra L. Tucker

Publication year - 2015

Publication title -

acs synthetic biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.156

H-Index - 66

ISSN - 2161-5063

DOI - 10.1021/acssynbio.5b00144

Subject(s) - peroxisome , microbiology and biotechnology , peroxisomal targeting signal , blue light , biology , biochemistry , chemistry , gene , physics , optics

The blue-light-responsive LOV2 domain of Avena sativa phototropin1 (AsLOV2) has been used to regulate activity and binding of diverse protein targets with light. Here, we used AsLOV2 to photocage a peroxisomal targeting sequence, allowing light regulation of peroxisomal protein import. We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light. This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.

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