Open AccessOptical Control of Peroxisomal Trafficking
Author(s) -
Jessica I. Spiltoir,
Devin Strickland,
Michael Glotzer,
Chandra L. Tucker
Publication year - 2015
Publication title -
acs synthetic biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.156
H-Index - 66
ISSN - 2161-5063
DOI - 10.1021/acssynbio.5b00144
Subject(s) - peroxisome , microbiology and biotechnology , peroxisomal targeting signal , blue light , biology , avena , chemistry , biochemistry , gene , physics , botany , optics
The blue-light-responsive LOV2 domain of Avena sativa phototropin1 (AsLOV2) has been used to regulate activity and binding of diverse protein targets with light. Here, we used AsLOV2 to photocage a peroxisomal targeting sequence, allowing light regulation of peroxisomal protein import. We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light. This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.