Open AccessSPaCE Swab: Point-of-Care Sensor for Simple and Rapid Detection of Acute Wound Infection
Author(s) -
Naing Tun Thet,
J. Mercer-Chalmers,
Rosemary Greenwood,
Amber Young,
Karen Coy,
Simon Booth,
Anthony Sack,
A. Toby A. Jenkins
Publication year - 2020
Publication title -
acs sensors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.055
H-Index - 57
ISSN - 2379-3694
DOI - 10.1021/acssensors.0c01265
Subject(s) - staphylococcus aureus , microbiology and biotechnology , pseudomonas aeruginosa , biofilm , candida albicans , pathogenic bacteria , biology , sepsis , virulence , enterococcus faecalis , bacteria , population , medicine , immunology , biochemistry , genetics , environmental health , gene
Wound infection is commonly observed after surgery and trauma but is difficult to diagnose and poorly defined in terms of objective clinical parameters. The assumption that bacteria in a wound correlate with infection is false; all wounds contain microorganisms, but not all wounds are clinically infected. This makes it difficult for clinicians to determine true wound infection, especially in wounds with pathogenic biofilms. If an infection is not properly treated, pathogenic virulence factors, such as rhamnolipids from Pseudomonas aeruginosa , can modulate the host immune response and cause tissue breakdown. Life-threatening sepsis can result if the organisms penetrate deep into host tissue. This communication describes the sensor development for five important clinical microbial pathogens commonly found in wounds: Staphylococcus aureus , P. aeruginosa , Candida albicans/auris, and Enterococcus faecalis (the SPaCE pathogens). The sensor contains liposomes encapsulating a self-quenched fluorescent dye. Toxins, expressed by SPaCE infecting pathogens in early-stage infected wounds, break down the liposomes, triggering dye release, thus changing the sensor color from yellow to green, an indication of infection. Five clinical species of bacteria and fungi, up to 20 strains each (totaling 83), were grown as early-stage biofilms in ex vivo porcine burn wounds. The biofilms were then swabbed, and the swab placed in the liposome suspension. The population density of selected pathogens in a porcine wound biofilm was quantified and correlated with colorimetric response. Over 88% of swabs switched the sensor on (10 7 -10 8 CFU/swab). A pilot clinical study demonstrated a good correlation between sensor switch-on and early-stage wound infection.