Open AccessDevelopment of a Smart Scaffold for Sequential Cancer Chemotherapy and Tissue Engineering
Author(s) -
Poulomi Sengupta,
Vinay Agrawal,
Bhagavatula L. V. Prasad
Publication year - 2020
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.9b03694
Subject(s) - scaffold , flow cytometry , drug delivery , cancer cell , biomedical engineering , tissue engineering , cell growth , nanotechnology , cisplatin , fluorescence microscope , chemistry , cell , fibroblast , biophysics , materials science , cancer , chemotherapy , fluorescence , in vitro , microbiology and biotechnology , medicine , biology , surgery , biochemistry , physics , quantum mechanics
The fabrication of a dual-functional drug-containing porous polymeric scaffold by layer-by-layer surface modification involving citrate-stabilized gold nanoparticles and cisplatin molecules is being reported. These scaffolds were characterized by electron microscopy and X-ray photoelectron spectroscopy. The capability of the scaffolds to release hydrated cisplatin in a slow and sustained manner over two days is established. Most importantly, the scaffolds turn nontoxic and cell-friendly after drug release, thus allowing the noncancerous fibroblast cells to adhere and proliferate (from 5000 cells to 16,000 cells in 6 days), becoming a potential solution toward an effective drug-carrying scaffold for volume-filling applications. The scaffold-mediated cancer cell killing and fibroblast cell proliferation were confirmed by fluorescence microscopy imaging, flow cytometry, and cell proliferation assays. We surmise that such a dual-purpose (drug-delivery and volume-filler) scaffold could help avoid the multiple surgical interventions needed for tumor surgery and cosmetic corrections. To the best of our knowledge, this is the first example of scaffolds with such a dual functionality which gets manifested in a sequential manner.