ESIPT Fluorescence Probe Based on Double-Switch Recognition Mechanism for Selective and Rapid Detection of Hydrogen Sulfide in Living Cells | Zendy

Hongwei Guan | Zendy; Aixia Zhang | Zendy; Peng Li | Zendy; Lixin Xia | Zendy; Feng Guo | Zendy

Open Access

ESIPT Fluorescence Probe Based on Double-Switch Recognition Mechanism for Selective and Rapid Detection of Hydrogen Sulfide in Living Cells

Author(s) -

Hongwei Guan,

Aixia Zhang,

Peng Li,

Lixin Xia,

Feng Guo

Publication year - 2019

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.9b00934

Subject(s) - benzothiazole , thiolysis , fluorescence , fluorophore , stokes shift , chemistry , photochemistry , bodipy , biophysics , biochemistry , antioxidant , polyphenol , physics , proanthocyanidin , quantum mechanics , biology

A novel fluorescence probe, HBTSeSe, was designed and synthesized for the detection of H 2 S with a double-switch mechanism of a broken diselenide bond followed by thiolysis of ether. Then, 2-(2'-hydroxyphenyl)benzothiazole (HBT) was released as fluorophore, which has large Stokes shift based on the excited state intramolecular proton transfer process. The probe responded selectively and rapidly to H 2 S, with the fluorescence increased by 47-fold immediately after the addition of H 2 S. HBTSeSe was able to detect H 2 S in the cytoplasm, specifically in cell imaging experiments. The results also showed that H 2 S was produced in the immune response of RAW264.7 cells activated by phorbol-12-myristate-13-acetate.

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