Open AccessSingle Platform for Gene and Protein Expression Analyses Using Luminescent Gold Nanoclusters
Author(s) -
Sunil Kumar Sailapu,
Deepanjalee Dutta,
Amaresh Kumar Sahoo,
Siddhartha Sankar Ghosh,
Arun Chattopadhyay
Publication year - 2018
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.7b01739
Subject(s) - nanoclusters , gene , recombinant dna , escherichia coli , hela , polymerase chain reaction , microbiology and biotechnology , target protein , dna , computational biology , biology , materials science , nanotechnology , biochemistry , cell
A single platform for gene and protein expression studies is proposed to pursue rapid diagnostics. A common method to synthesize gold (Au) nanoclusters on both DNA and protein template was developed using a benchtop device. The method of synthesis is rapid and versatile and can be applied to different classes of DNA/protein. Employing luminescent Au nanoclusters as the signal-generating agents, the device enables carrying out reverse transcriptase polymerase chain reaction and array-based analyses of multiple genes/proteins simultaneously using switchable holders and custom-designed software. The device and methods were applied to evaluate gene profiling related to apoptosis in HeLa cancer cells and further to analyze the protein expressions of glutathione- S -transferase (GST) and GST-tagged human granulocyte macrophage colony-stimulating factor (GST-hGMCSF) recombinant proteins purified from bacterial strains of BL21(DE3) Escherichia coli ( E. coli ). The device with user-friendly methods for diagnosis using the luminescence of Au nanoclusters offers potential use in disease diagnostics with a vision to extend health care facilities especially to remote geographical locations.