Open AccessMultiresidue Method for the Rapid Determination of Pesticide Residues in Tea Using Ultra Performance Liquid Chromatography Orbitrap High Resolution Mass Spectrometry and In-Syringe Dispersive Solid Phase Extraction
Author(s) -
Hongping Chen,
Guanwei Gao,
Yunfeng Chai,
Guicen Ma,
Zhenxia Hao,
Chen Wang,
Xin Liu,
Chengyin Lu
Publication year - 2017
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.7b00863
Subject(s) - orbitrap , chromatography , mass spectrometry , solid phase extraction , chemistry , high performance liquid chromatography , extraction (chemistry) , liquid chromatography–mass spectrometry , analytical chemistry (journal)
A method based on in-syringe dispersive solid phase extraction (IS-D-SPE) and ultra performance liquid chromatography Orbitrap high resolution mass spectrometry for the multiresidue analysis of 117 pesticides in tea was developed. Full scan mode was acquired over an m / z range of 100-800 with Orbitrap resolution at 70000, followed by full scan/dd-MS 2 mode for confirmation. The identification criteria of retention time and mass accuracy tolerance was ±0.20 min and ±5.0 ppm, respectively. MS/MS fragment ions obtained dd-MS 2 were necessary to identify the pesticides with the same molecular mass weight. The IS-D-SPE technique involved a mixture of 200 mg PSA, 100 mg C18, and 15 mg multiwalled carbon nanotubes for the cleanup of tea matrix. Good linearity ( R 2 > 0.99) for 117 pesticides was obtained. Satisfactory recoveries in the range of 70-120% were obtained for 105 pesticides, while intraday and interday precisions were below 20%. Limits of quantification were generally 10 μg kg -1 . Finally, this method was employed to analyze 117 pesticides in 70 tea samples.