Open AccessMutagenesis Reveals an Unusual Combination of Guanines in RNA G-Quadruplex Formation
Author(s) -
Prachi Agarwala,
Gargi Pal,
Satyaprakash Pandey,
Souvik Maiti
Publication year - 2017
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.7b00377
Subject(s) - mutagenesis , chemistry , rna , g quadruplex , site directed mutagenesis , computational biology , microbiology and biotechnology , biochemistry , dna , biology , mutation , mutant , gene
The classic G-quadruplex motif consists of a continuous array of 3-4 guanine residues with an intermittent loop size of 1-7 nucleotides (G 3-4 N 1-7 G 3-4 N 1-7 G 3-4 N 1-7 G 3-4 ). An RNA G-quadruplex is able to attain only one parallel G-quadruplex topology owing to steric constraints. Investigating the possibilities of the formation of RNA G-quadruplexes with a stretch of sequences deviating from this classic motif will add to the overall conformations of RNA G-quadruplexes, bestowing diversity to this structure. Here, we report unusual combinations of guanine residues involved in RNA G-quadruplex formation in the 5' untranslated region (UTR) of the von Willebrand factor (VWF) mRNA using the mutagenesis approach. Different permutations and combinations of guanine residues form G-quadruplexes. Upon investigation, G-quadruplexes in 5' UTR of VWF mRNA are shown to exhibit an inhibitory function.