Dispersed Gold Nanoparticle Array Produced by Apoferritins Utilizing Biomineralization and Chemical Conversion | Zendy

Kazuyuki Nobusawa | Zendy; Naofumi Okamoto | Zendy; Karen Siew Ling Chong | Zendy; Xi Lin | Zendy; Kenji Iwahori | Zendy; Ichiro Yamashita | Zendy

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Dispersed Gold Nanoparticle Array Produced by Apoferritins Utilizing Biomineralization and Chemical Conversion

Author(s) -

Kazuyuki Nobusawa,

Naofumi Okamoto,

Karen Siew Ling Chong,

Xi Lin,

Kenji Iwahori,

Ichiro Yamashita

Publication year - 2017

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.6b00550

Subject(s) - biomineralization , nanoparticle , nanotechnology , colloidal gold , materials science , chemical engineering , engineering

A new method for producing a dispersed gold nanoparticle (Au NP) array to anchor probe DNAs onto a DNA-sensing electrode has been developed. A homogenous gold sulfide (Au 2 S) core (precursor of Au NP) was biomineralized in the cavity of a mutant apoferritin (K98E) with enhanced negative outer-surface charges. We employed a slow chemical reaction system utilizing a stable cationic gold complex. K98E could attract the gold complex, and Au 2 S NPs were synthesized. K98E enabled dispersed placement of the synthesized Au 2 S core onto a cationic 3-aminopropyltriethoxysilane (APTES) layer on a substrate. UV-ozone treatment eliminated the protein shells and APTES layer. X-ray photoelectron spectroscopy confirmed that the Au 2 S core was reduced to Au NPs under the same treatment. Atomic force microscopy (AFM) clearly showed that the combination of apoferritin versatility, chemical system design, and UV-ozone treatment successfully produced a dispersed Au NP array on the substrate.

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