Open AccessConformational Changes in High-Density Lipoprotein Nanoparticles Induced by High Payloads of Paramagnetic Lipids
Author(s) -
Pedro RamosCabrer,
François Fay,
Brenda L. Sánchez-Gaytán,
Jun Tang,
José Castillo,
Zahi A. Fayad,
Willem J. M. Mulder
Publication year - 2016
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.6b00108
Subject(s) - gadolinium , nanoparticle , chemistry , molar ratio , lipoprotein , in vivo , paramagnetism , high density lipoprotein , cholesterol , biophysics , nuclear magnetic resonance , materials science , nanotechnology , biochemistry , organic chemistry , physics , microbiology and biotechnology , quantum mechanics , biology , catalysis
High-density lipoprotein (HDL) nanoparticles doped with gadolinium lipids can be used as magnetic resonance imaging diagnostic agents for atherosclerosis. In this study, HDL nanoparticles with different molar fractions of gadolinium lipids (0 < x Gd-lipids < 0.33) were prepared, and the MR relaxivity values ( r 1 and r 2) for all compositions were measured. Both r 1 and r 2 parameters reached a maximal value at a molar fraction of approximately x Gd-lipids = 0.2. Higher payloads of gadolinium did not significantly increase relaxivity values but induced changes in the structure of HDL, increasing the size of the particles from d H = 8.2 ± 1.6 to 51.7 ± 7.3 nm. High payloads of gadolinium lipids trigger conformational changes in HDL, with potential effects on the in vivo behavior of the nanoparticles.