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Characterization of carbon black (CB) nanomaterials is required in industrial and research areas. Hence, in this study, asymmetrical flow field flow fractionation coupled to UV-vis and DLS detectors in series (AF4-UV-vis-DLS) was studied to evaluate the CB dispersion behavior in polymeric and biological dispersants, given the relevance of these media in practical applications. Under the experimental conditions, the results indicated that polymeric and biological dispersions showed size distributions with hydrodynamic diameters of 404 and 175 nm, respectively, for a particle core diameter of 40 nm. The polymeric dispersant provided lower stability as a function of time than that achieved by the biological dispersant. AF4 allowed separation of different core-sized CB (40, 69, and 72 nm) according to their hydrodynamic size using cross-flow rates of 0.5 mL·min -1 and 1 mL·min -1 for polymeric and biological dispersants, respectively. The dilution of the polymeric dispersion with different real water matrices produced a dramatic loss of dispersion stability, this effect being negligible in the case of biological dispersions.

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Characterization and Quantitation of Carbon Black Nanomaterials in Polymeric and Biological Aqueous Dispersants by Asymmetrical Flow Field Flow Fractionation