Open AccessDetection of Listeria monocytogenes Using Luminol-Functionalized AuNF-Labeled Aptamer Recognition and Magnetic Separation
Author(s) -
Weifeng Chen,
Liwei Cui,
Yanyan Song,
Wei Chen,
Yuan Su,
Weidan Chang,
Wentao Xu
Publication year - 2021
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.1c03527
Subject(s) - aptamer , luminol , listeria monocytogenes , chemiluminescence , chemistry , linear range , linear relationship , chromatography , signal (programming language) , detection limit , microbiology and biotechnology , biology , computer science , mathematics , bacteria , genetics , statistics , programming language
A capture probe was constructed using a combination of magnetic Fe 3 O 4 nanoparticles and an aptamer directed toward Listeria monocytogenes . A signal probe was prepared by combining luminol-functionalized flowerlike gold nanoparticles, obtained by combining luminol with chitosan bearing a complementary sequence of the aptamer. The complex consisting of the capture probe and signal probe could be removed through magnetic separation. Where the target was present within a sample, it competed with the complementary sequence for binding to the aptamer, causing a change of the chemiluminescent signal. The results indicated that a good linear relationship existed over the concentration range 1.0 × 10 1 -1.0 × 10 5 CFU·mL -1 . It was established that it was feasible to use this approach to detect L. monocytogenes at levels as low as 6 CFU·mL -1 in milk samples.