Open AccessIsolation, Purification, Characterization, and Immunomodulatory Activity Analysis of α-Glucans from Spirulina platensis
Author(s) -
Jian Li,
Yaqi Zhang,
Yang Shen,
Zhenhua Lü,
Guiling Li,
Jingwen Li,
Bo Zhou,
Daren Wu,
Li Wang
Publication year - 2021
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.1c02175
Subject(s) - spirulina (dietary supplement) , monosaccharide , polysaccharide , chemistry , sephadex , rhamnose , chromatography , high performance liquid chromatography , dextran , biochemistry , cellulose , composition (language) , fractionation , enzyme , organic chemistry , raw material , linguistics , philosophy
Crude polysaccharides from Spirulina platensis (SP) were isolated by maceration with a hot alkali solution and further fractionated by DEAE-52 cellulose and Sephadex G-100 chromatography into two purified fractions PSP-1 and PSP-2. The monosaccharide composition analysis indicated that SP was mainly composed of rhamnose and glucose, while PSP-1 and PSP-2 were composed only of glucose. The composition analysis of PSP-1 and PSP-2 by HPLC, FT-IR, and NMR showed that PSP-1 and PSP-2 were branching dextran, and their structures were (1 → 4)-linked-α-D-Glcp as the main chain, and C-6 replaced the single α-D-Glcp as the linear structure of the branch chain. The glucans (SP/PSP-1/PSP-2) can significantly improve the phagocytic ability of macrophages, enhance iNOS activity, promote NO production, and increase IL-6 mRNA expression, so they may possess certain immunomodulatory activity.