Open AccessUltrasensitive Detection of Nasopharyngeal Carcinoma-Related MiRNA through Garland Rolling Circle Amplification Integrated Catalytic Hairpin Assembly
Author(s) -
Shayue Xu,
Zhiping Lou
Publication year - 2021
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.1c00154
Subject(s) - nasopharyngeal carcinoma , rolling circle replication , microrna , biomarker , sensitivity (control systems) , signal (programming language) , computational biology , biology , computer science , gene , medicine , genetics , electronic engineering , radiation therapy , dna replication , engineering , programming language
MiRNA is reported to be closely related to nasopharyngeal carcinoma and has the potential to be a biomarker for the early diagnosis of nasopharyngeal carcinoma. However, the detection of miRNAs remains to be improved, given their complexity and low sensitivity. Herein, we propose here a novel miRNA detection method through the integration of garland RCA and CHA. In detail, the method is composed of two important signal amplification processes. For the first signal amplification process, the target miRNA could initiate garland RCA and then generate a nicking site on the products with the assistance of Nb.BbvCI enzymes. Afterward, a CHA process is induced with a designed H probe through the two signal amplification processes; the method exhibited a much-improved sensitivity. At last, we believe that this method is a promising approach capable of being applied in screening, diagnosing, and prognosticating multiple diseases.