Open AccessMagnesium Ammonium Phosphate Composite Cell-Laden Hydrogel Promotes Osteogenesis and Angiogenesis In Vitro
Author(s) -
Chang Liu,
Guangzheng Yang,
Mingliang Zhou,
Xiangkai Zhang,
Xiaolin Wu,
Pei Wu,
Xiaoyu Gu,
Xinquan Jiang
Publication year - 2021
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.0c06083
Subject(s) - struvite , chemistry , angiogenesis , self healing hydrogels , umbilical vein , dental pulp stem cells , biomedical engineering , gelatin , regeneration (biology) , phosphate , microbiology and biotechnology , in vitro , biochemistry , polymer chemistry , medicine , biology , cancer research
Injectable hydrogels provide an effective strategy for minimally invasive treatment on irregular bony defects in the maxillofacial region. To improve the osteoinduction of gelatin methacrylate (GelMA), we fabricated a three-dimensional (3D) culture system based on the incorporation of magnesium ammonium phosphate hexahydrate (struvite) into GelMA. The optimal concentration of struvite was investigated using the struvite extracts, and 500 μg mL -1 was found to be the most suitable concentration for the osteogenesis of dental pulp stem cells (DPSCs) and angiogenesis of human umbilical vein endothelial cells (HUVECs). We prepared the GelMA composite (MgP) with 500 μg mL -1 struvite. Struvite did not affect the cross-linking of GelMA and released Mg 2+ during degradation. The cell delivery system using MgP improved the laden-cell viability, upregulated the expression of osteogenic and angiogenic-differentiation-related genes, and promoted cell migration. Overall, the modifications made to the GelMA in this study improved osteoinduction and demonstrated great potential for application in vascularized bone tissue regeneration.