Open AccessInhibition of Enterovirus 71 by Selenium Nanoparticles Loaded with siRNA through Bax Signaling Pathways
Author(s) -
Zhengfang Lin,
Yinghua Li,
Tiantian Xu,
Min Guo,
Changbing Wang,
Mingqi Zhao,
Haiyang Chen,
Jianling Kuang,
Wanling Li,
Yingying Zhang,
Tao Lin,
Yi Chen,
Huanhui Chen,
Bing Zhu
Publication year - 2020
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.0c01382
Subject(s) - small interfering rna , enterovirus 71 , polyethylenimine , rna interference , apoptosis , cell , chemistry , cell culture , microbiology and biotechnology , virus , rna , virology , enterovirus , biology , gene , transfection , biochemistry , genetics
Enterovirus 71 (EV71) is the principal pathogen leading to severe cases of hand, foot, and mouth disease (HFMD). Specific drugs for EV71 are not discovered currently. Small interfering RNA (siRNA) provides a promising antiviral treatment pathway, but it is difficult to cross cell membranes and is easy to degrade. Nanoparticles are promising for their carrying capacity currently. In this study, the siRNA targeting EV71 VP1 gene was loaded with selenium nanoparticles (SeNPs) and surface decorated with polyethylenimine (PEI) (Se@PEI@siRNA). Se@PEI@siRNA showed a remarkable interference efficiency in the nerve cell line SK-N-SH and prevented the cells to be infected. The mechanism study revealed that Se@PEI@siRNA could lighten the extent of SK-N-SH cells for staying in the sub-G1 phase. Activation of Bax apoptosis signaling was restrained either. Taken together, this study demonstrated that Se@PEI@siRNA is a promising drug against EV71 virus.