Modifying Membrane Morphology and Interactions with DNA Origami Clathrin-Mimic Networks | Zendy

Céline M. A. Journot | Zendy; Vivek Ramakrishna | Zendy; Mark I. Wallace | Zendy; Andrew J. Turberfield | Zendy

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Modifying Membrane Morphology and Interactions with DNA Origami Clathrin-Mimic Networks

Author(s) -

Céline M. A. Journot,

Vivek Ramakrishna,

Mark I. Wallace,

Andrew J. Turberfield

Publication year - 2019

Publication title -

acs nano

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.554

H-Index - 382

eISSN - 1936-086X

pISSN - 1936-0851

DOI - 10.1021/acsnano.8b07734

Subject(s) - clathrin , lipid bilayer , vesicle , dna origami , biophysics , dna nanotechnology , polymerization , membrane , bilayer , nanotechnology , chemistry , dna , materials science , nanostructure , biology , biochemistry , polymer , organic chemistry

We describe the triggered assembly of a bioinspired DNA origami meshwork on a lipid membrane. DNA triskelia, three-armed DNA origami nanostructures inspired by the membrane-modifying protein clathrin, are bound to lipid mono- and bilayers using cholesterol anchors. Polymerization of triskelia, triggered by the addition of DNA staples, links triskelion arms to form a mesh. Using transmission electron microscopy, we observe nanoscale local deformation of a lipid monolayer induced by triskelion polymerization that is reminiscent of the formation of clathrin-coated pits. We also show that the polymerization of triskelia bound to lipid bilayers modifies interactions between them, inhibiting the formation of a synapse between giant unilamellar vesicles and a supported lipid bilayer.

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