Open AccessExploring Ligand-Directed N-Acyl-N-alkylsulfonamide-Based Acylation Chemistry for Potential Targeted Degrader Development
Author(s) -
Mingxing Teng,
Jie Jiang,
Scott B. Ficarro,
Hyuk-Soo Seo,
Jae Hyun Bae,
Katherine A. Donovan,
Eric S. Fischer,
Tinghu Zhang,
Sirano DhePaga,
Jarrod A. Marto,
Nathanael S. Gray
Publication year - 2021
Publication title -
acs medicinal chemistry letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 66
ISSN - 1948-5875
DOI - 10.1021/acsmedchemlett.1c00285
Subject(s) - bioconjugation , acylation , chemistry , ligand (biochemistry) , combinatorial chemistry , chemical biology , target protein , biophysics , computational biology , biochemistry , receptor , biology , gene , catalysis
Ligand-directed bioconjugation strategies have been used for selective protein labeling in live cells or tissue samples in applications such as live-cell imaging. Here we hypothesized that a similar strategy could be used for targeted protein degradation. To test this possibility, we developed a series of CDK2-targeting N -acyl- N -alkylsulfonamide (NASA)-containing acylation probes. The probes featured three components: a CDK2 homing ligand, a CRL4 CRBN E3 ligase recruiting ligand, and a NASA functionality. We determined that upon target binding, NASA-mediated reaction resulted in selective functionalization of Lys89 on purified or native CDK2. However, we were unable to observe CDK2 degradation, which is in contrast to the efficient degradation achieved by the use of a structurally similar reversible bivalent degrader. Our analysis suggests that the lack of degradation is due to the failure to form a productive CDK2:CRBN complex. Therefore, although this work demonstrates that NASA chemistry can be used for protein labeling, whether this strategy could enable efficient protein degradation remains an open question.