Open AccessA Single Tool to Monitor Multiple Protein–Protein Interactions of the Escherichia coli Acyl Carrier Protein
Author(s) -
Katherine Charov,
Michael D. Burkart
Publication year - 2019
Publication title -
acs infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.324
H-Index - 39
ISSN - 2373-8227
DOI - 10.1021/acsinfecdis.9b00150
Subject(s) - allosteric regulation , escherichia coli , protein–protein interaction , acyl carrier protein , drug discovery , computational biology , biology , target protein , enzyme , biochemistry , chemistry , gene
Protein-protein interactions are ubiquitous to all domains of life and have gained recent interest as drug targets. However, many current methods to study protein-protein interactions can be costly and are low-throughput. Here, we demonstrate a solvatochromic tool based on the natural post-translational modification of the Escherichia coli acyl carrier protein (EcACP) used to visualize protein-protein interactions between EcACP and 13 different partner enzymes from several biosynthetic pathways. We use this tool to confirm proposed interactions between EcACP and both catalytic and regulatory proteins. We also show the utility of this method toward detecting allosteric changes to partner enzyme structure and the validation of active site inhibitors. We anticipate the future adaptation of this assay into a high-throughput screen for antibiotic discovery.