Open AccessExpanding the Chemical Diversity of Genetically Encoded Libraries
Author(s) -
Sabrina E. Iskandar,
Victoria A. Haberman,
A. Bowers
Publication year - 2020
Publication title -
acs combinatorial science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 81
eISSN - 2156-8952
pISSN - 2156-8944
DOI - 10.1021/acscombsci.0c00179
Subject(s) - genetic code , computational biology , directed molecular evolution , selection (genetic algorithm) , ribosome , genetically modified organism , yeast , directed evolution , chemistry , phage display , genetics , biology , gene , computer science , rna , artificial intelligence , mutant , antibody
The power of ribosomes has increasingly been harnessed for the synthesis and selection of molecular libraries. Technologies, such as phage display, yeast display, and mRNA display, effectively couple genotype to phenotype for the molecular evolution of high affinity epitopes for many therapeutic targets. Genetic code expansion is central to the success of these technologies, allowing researchers to surpass the intrinsic capabilities of the ribosome and access new, genetically encoded materials for these selections. Here, we review techniques for the chemical expansion of genetically encoded libraries, their abilities and limits, and opportunities for further development. Importantly, we also discuss methods and metrics used to assess the efficiency of modification and library diversity with these new techniques.