Open AccessEthanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry
Author(s) -
Aurélie Roux,
Shelley N. Jackson,
Ludovic Muller,
Damon C. Barbacci,
Joseph O’Rourke,
Panayotis K. Thanos,
Nora D. Volkow,
Carey D. Balaban,
J. Schultz,
Amina S. Woods
Publication year - 2016
Publication title -
acs chemical neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.158
H-Index - 69
ISSN - 1948-7193
DOI - 10.1021/acschemneuro.6b00120
Subject(s) - lipid metabolism , sphingolipid , ethanol , chemistry , medicine , alcohol , lipidomics , endocrinology , biochemistry , biology
Alcohol abuse is a chronic disease characterized by the consumption of alcohol at a level that interferes with physical and mental health and causes serious and persistent changes in the brain. Lipid metabolism is of particular interest due to its high concentration in the brain. Lipids are the main component of cell membranes, are involved in cell signaling, signal transduction, and energy storage. In this study, we analyzed lipid composition of chronically ethanol exposed mouse brains. Juvenile (JUV) and adult (ADU) mice were placed on a daily limited-access ethanol intake model for 52 days. After euthanasia, brains were harvested, and total lipids were extracted from brain homogenates. Samples were analyzed using high resolution mass spectrometry and processed by multivariate and univariate statistical analysis. Significant lipid changes were observed in different classes including sphingolipids, fatty acids, lysophosphatidylcholines, and other glycerophospholipids.