Collagen Type I and II Blend Hydrogel with Autologous Mesenchymal Stem Cells as a Scaffold for Articular Cartilage Defect Repair | Zendy

Claire E. Kilmer | Zendy; Carly M. Battistoni | Zendy; Abigail Cox | Zendy; Gert J. Breur | Zendy; Alyssa Panitch | Zendy; Julie C. Liu | Zendy

Open Access

Collagen Type I and II Blend Hydrogel with Autologous Mesenchymal Stem Cells as a Scaffold for Articular Cartilage Defect Repair

Author(s) -

Claire E. Kilmer,

Carly M. Battistoni,

Abigail Cox,

Gert J. Breur,

Alyssa Panitch,

Julie C. Liu

Publication year - 2020

Publication title -

acs biomaterials science and engineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.082

H-Index - 50

ISSN - 2373-9878

DOI - 10.1021/acsbiomaterials.9b01939

Subject(s) - self healing hydrogels , chondrogenesis , mesenchymal stem cell , cartilage , type ii collagen , chondrocyte , glycosaminoglycan , chemistry , alkaline phosphatase , biomedical engineering , tissue engineering , microbiology and biotechnology , materials science , anatomy , medicine , biology , biochemistry , polymer chemistry , enzyme

Collagen type II is a promising material to repair cartilage defects since it is a major component of articular cartilage and plays a key role in chondrocyte function. This study investigated the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (MSCs) embedded within a 3:1 collagen type I to II blend (Col I/II) hydrogel or an all collagen type I (Col I) hydrogel. Glycosaminoglycan (GAG) production in Col I/II hydrogels was statistically higher than that in Col I hydrogels or pellet culture, and these results suggested that adding collagen type II promoted GAG production. Col I/II hydrogels had statistically lower alkaline phosphatase (AP) activity than pellets cultured in a chondrogenic medium. The ability of MSCs encapsulated in Col I/II hydrogels to repair cartilage defects was investigated by creating two cartilage defects in the femurs of rabbits. After 13 weeks, histochemical staining suggested that Col I/II blend hydrogels provided favorable conditions for cartilage repair. Histological scoring revealed a statistically higher cartilage repair score for the Col I/II hydrogels compared to either the Col I hydrogels or empty defect controls. Results from this study suggest that there is clinical value in the cartilage repair capabilities of our Col I/II hydrogel with encapsulated MSCs.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research