Open AccessEvaluation of the Osteoinductive Capacity of Polydopamine-Coated Poly(ε-caprolactone) Diacrylate Shape Memory Foams
Author(s) -
Joshua Erndt-Marino,
Dany J. MunozPinto,
Satyavrata Samavedi,
Andrea C. JimenezVergara,
Patricia DíazRodríguez,
Lindsay N. Woodard,
Dawei Zhang,
Melissa A. Grunlan,
Mariah S. Hahn
Publication year - 2015
Publication title -
acs biomaterials science and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.082
H-Index - 50
ISSN - 2373-9878
DOI - 10.1021/acsbiomaterials.5b00445
Subject(s) - mesenchymal stem cell , chondrogenesis , caprolactone , adipogenesis , osteopontin , materials science , runx2 , alkaline phosphatase , biomedical engineering , polymer , coating , chemistry , composite material , copolymer , biochemistry , immunology , pathology , medicine , enzyme
Recently, a novel shape memory polymer foam based on the photopolymerization of poly( ε -caprolactone) diacrylate (PCLDA) has been developed. These PCLDA foams enter a temporary softened state when briefly treated with warm saline ( T saline > T m of PCLDA), allowing them to conform to irregular bone defect "boundaries" prior to shape setting. When coated with a mechanically stable polydopamine (PD) layer, these PCLDA foams have previously been demonstrated to induce hydroxyapatite deposition. In the present study, the osteoinductivity of these "self-fitting" PD-coated PCLDA (PD-PCLDA) materials was evaluated relative to uncoated PCLDA (U-PCLDA) controls using bone marrow-derived human mesenchymal stem cells (h-MSCs). When cultured in the absence of osteogenic media supplements, PD-PCLDA scaffolds expressed similar levels of Runx2, alkaline phosphatase, and osteopontin protein as U-PCLDA scaffolds cultured in the presence of osteogenic media supplements. In addition, PD-PCLDA scaffolds cultured without osteogenic supplements did not significantly promote undesired lineage progression (e.g., adipogenesis or chondrogenesis) of h-MSCs. Cumulatively, these data indicate that PD-PCLDA materials display increased osteoinductivity relative to U-PCLDA substrates. Future studies will examine tethered osteogenic factors or peptides toward augmenting the osteoinductive properties of the PD-PCLDA foams.