DNA-Coated Gold Nanoparticles for the Detection of mRNA in Live Hydra Vulgaris Animals | Zendy

María Moros | Zendy; Maria-Eleni Kyriazi | Zendy; Afaf H. ElSagheer | Zendy; Tom Brown | Zendy; Claudia Tortiglione | Zendy; Antonios G. Kanaras | Zendy

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DNA-Coated Gold Nanoparticles for the Detection of mRNA in Live Hydra Vulgaris Animals

Author(s) -

María Moros,

Maria-Eleni Kyriazi,

Afaf H. ElSagheer,

Tom Brown,

Claudia Tortiglione,

Antonios G. Kanaras

Publication year - 2018

Publication title -

acs applied materials and interfaces

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.535

H-Index - 228

eISSN - 1944-8252

pISSN - 1944-8244

DOI - 10.1021/acsami.8b17846

Subject(s) - lernaean hydra , in vivo , oligonucleotide , colloidal gold , in vitro , microbiology and biotechnology , biology , messenger rna , nanoparticle , dna , intracellular , nanotechnology , materials science , biophysics , gene , biochemistry , genetics

Advances in nanoparticle design have led to the development of nanoparticulate systems that can sense intracellular molecules, alter cellular processes, and release drugs to specific targets in vitro. In this work, we demonstrate that oligonucleotide-coated gold nanoparticles are suitable for the detection of mRNA in live Hydra vulgaris, a model organism, without affecting the animal's integrity. We specifically focus on the detection of Hymyc1 mRNA, which is responsible for the regulation of the balance between stem cell self-renewal and differentiation. Myc deregulation is found in more than half of human cancers, thus the ability to detect in vivo related mRNAs through innovative fluorescent systems is of outmost interest.

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