Open AccessSelf-Assembled DNA Immunonanoflowers as Multivalent CpG Nanoagents
Author(s) -
Liqin Zhang,
Guizhi Zhu,
Lei Mei,
Cuichen Wu,
Liping Qiu,
Cheng Cui,
Yuan Liu,
I-Ting Teng,
Weihong Tan
Publication year - 2015
Publication title -
acs applied materials and interfaces
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1944-8252
pISSN - 1944-8244
DOI - 10.1021/acsami.5b06987
Subject(s) - cpg site , cpg oligodeoxynucleotide , nuclease , rolling circle replication , dna , tumor necrosis factor alpha , immune system , biology , guanine , cancer cell , materials science , dna methylation , microbiology and biotechnology , cancer research , cancer , dna replication , immunology , biochemistry , gene , nucleotide , genetics , gene expression
Synthetic unmethylated cytosine-guanine (CpG) oligodeoxynucleotides are immunostimulatory motifs that have shown promise as vaccines or adjuvants for diseases such as cancers and infectious diseases. In the present work, novel immuno-nanoflowers (NFs), self-assembled from long DNA integrated with tandem CpG through rolling circle replication, were developed for efficient CpG delivery and protection from nuclease degradation. In a model of macrophage-like cells, the CpG NFs proved to be potent immunostimulators by triggering the proliferation of these immune cells, which, in turn, secreted immunostimulatory cytokines, including tumor necrosis factor α, interleukin-6, and interleukin-10. These results demonstrate the ability of CpG NFs to induce cancer cell apoptosis and necrosis.