Open AccessProtective Polymer Coatings for High-Throughput, High-Purity Cellular Isolation
Author(s) -
Gabriela Romero,
Jacob J. Lilly,
Nathan S. Abraham,
Hainsworth Y. Shin,
Vivek Balasubramaniam,
Tohru Izumi,
Brad J. Berron
Publication year - 2015
Publication title -
acs applied materials and interfaces
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 2.535
H-Index - 228
eISSN - 1944-8252
pISSN - 1944-8244
DOI - 10.1021/acsami.5b06298
Subject(s) - lysis , materials science , polymer , coating , cell , yield (engineering) , pulmonary surfactant , nanotechnology , biology , microbiology and biotechnology , biochemistry , metallurgy , composite material
Cell-based therapies are emerging as the next frontier of medicine, offering a plausible path forward in the treatment of many devastating diseases. Critically, current methods for antigen positive cell sorting lack a high throughput method for delivering ultrahigh purity populations, prohibiting the application of some cell-based therapies to widespread diseases. Here we show the first use of targeted, protective polymer coatings on cells for the high speed enrichment of cells. Individual, antigen-positive cells are coated with a biocompatible hydrogel which protects the cells from a surfactant solution, while uncoated cells are immediately lysed. After lysis, the polymer coating is removed through orthogonal photochemistry, and the isolate has >50% yield of viable cells and these cells proliferate at rates comparable to control cells. Minority cell populations are enriched from erythrocyte-depleted blood to >99% purity, whereas the entire batch process requires 1 h and <$2000 in equipment. Batch scale-up is only contingent on irradiation area for the coating photopolymerization, as surfactant-based lysis can be easily achieved on any scale.