Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes | Zendy

Luca Mascheroni | Zendy; Valentina Francia | Zendy; Beatrice Rossotti | Zendy; Elisabetta Ranucci | Zendy; Paolo Ferruti | Zendy; Daniela Maggioni | Zendy; Anna Salvati | Zendy

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Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes

Author(s) -

Luca Mascheroni,

Valentina Francia,

Beatrice Rossotti,

Elisabetta Ranucci,

Paolo Ferruti,

Daniela Maggioni,

Anna Salvati

Publication year - 2020

Publication title -

acs applied materials and interfaces

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.535

H-Index - 228

eISSN - 1944-8252

pISSN - 1944-8244

DOI - 10.1021/acsami.0c08033

Subject(s) - nucleus , endocytosis , nanocarriers , ruthenium , biophysics , cytosol , endosome , materials science , singlet oxygen , cell , nanotechnology , photochemistry , chemistry , oxygen , drug delivery , microbiology and biotechnology , biochemistry , catalysis , biology , organic chemistry , enzyme

Strategies for endosomal escape and access to the cell nucleus are highly sought for nanocarriers to deliver their load efficiently following endocytosis. In this work, we have studied the uptake and intracellular trafficking of a polycationic polyamidoamine (PAA) endowed with a luminescent Ru complex, Ru-PhenAN, that shows unique trafficking to the cell nucleus. Live cell imaging confirmed the capacity of this polymer to access the nucleus, excluding artifacts due to cell fixation, and clarified that the mechanism of escape is light-triggered and relies on the presence of the Ru complexes and their capacity to absorb light and act as photosensitizers for singlet oxygen production. These results open up the possibility to use PAA-ruthenium complexes for targeted light-triggered delivery of genetic material or drugs to the cytosol and nucleus.

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