Open AccessLigand Guided Selection (LIGS) of Artificial Nucleic Acid Ligands against Cell Surface Targets
Author(s) -
Hasan Zumrut,
Prabodhika Mallikaratchy
Publication year - 2019
Publication title -
acs applied bio materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.764
H-Index - 17
ISSN - 2576-6422
DOI - 10.1021/acsabm.9b00938
Subject(s) - aptamer , systematic evolution of ligands by exponential enrichment , nucleic acid , computational biology , selex aptamer technique , rna , ligand (biochemistry) , dna , small molecule , biology , chemistry , combinatorial chemistry , biochemistry , receptor , gene , genetics
With the success of RNA-based therapeutic drugs, the demand has increased for sophisticated nucleic-acid-based targeting agents. Nucleic acid aptamers (NAAs), in this regard, represent a suitable class of molecules with synthetic versatility. Aptamers are composed of single-stranded RNA/DNA/XNA molecules, which can be identified using a method called systematic evolution of ligands by exponential enrichment (SELEX) against any molecule. This Spotlight summarizes the recent introduction of ligand guided selection (LIGS), which will permit the identification of a wide range of functional aptamers against complex targets such as cell surface receptors while maintaining their native functional state. Aptamers identified from LIGS will allow researchers to develop aptamers in biomedicine as low-cost, stable therapeutic agents and diagnostic molecules or biochemical devices.