Open AccessDendritic Hydrogel Bioink for 3D Printing of Bacterial Microhabitat
Author(s) -
Partha Sarathi Sheet,
Dipankar Koley
Publication year - 2019
Publication title -
acs applied bio materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.764
H-Index - 17
ISSN - 2576-6422
DOI - 10.1021/acsabm.9b00866
Subject(s) - sonication , confocal laser scanning microscopy , chemistry , bacteria , chemical engineering , bacterial growth , nanotechnology , microscopy , materials science , biophysics , chromatography , biology , physics , optics , engineering , genetics
A glucose-modified dendritic hydrogel is used as a bioink for bacterial encapsulation. This biocompatible hydrogel is a potentially suitable alternative to conventional alginate hydrogel for bacterial encapsulation, as it readily forms gel in the presence of Na + or K + ions without any additional stimuli such as pH, temperature, sonication, or the presence of divalent metal ions. We created a bacterial microhabitat by adding the gelator to phosphate-buffered saline containing live bacteria at physiological pH and using an additive three-dimensional (3D) printing technique. The bacteria remained viable and metabolically active within the 3D printed bacterial microhabitat, as shown with confocal laser scanning microscopy (CLSM) and scanning electrochemical microscopy (SECM).