Open AccesspH-Responsive, Adorned Nanoniosomes for Codelivery of Cisplatin and Epirubicin: Synergistic Treatment of Breast Cancer
Author(s) -
Ali Moammeri,
Koorosh Abbaspour,
Alireza Zafarian,
Elham Jamshidifar,
Hamidreza Motasadizadeh,
Farnaz Dabbagh Moghaddam,
Zeinab Salehi,
Pooyan Makvandi,
Rassoul Dinarvand
Publication year - 2022
Publication title -
acs applied bio materials
Language(s) - English
Resource type - Journals
ISSN - 2576-6422
DOI - 10.1021/acsabm.1c01107
Subject(s) - skbr3 , nanocarriers , breast cancer , niosome , apoptosis , pharmacology , in vivo , chemistry , cancer research , epirubicin , cisplatin , cancer , medicine , chemotherapy , biology , biochemistry , drug , microbiology and biotechnology , vesicle , human breast , membrane
Combination chemotherapy has become a treatment modality for breast cancer. However, serious side effects and high cytotoxicity associated with this combination therapy make it a high-risk method for breast cancer treatment. This study evaluated the anticancer effect of decorated niosomal nanocarriers loaded with cisplatin (CIS) and epirubicin (EPI) in vitro (on SKBR3 and 4T1 breast cancer cells) and in vivo on BALB/c mice. For this purpose, polyethylene glycol (PEG) and folic acid (FA) were employed to prepare a functionalized niosomal system to improve endocytosis. FA-PEGylated niosomes exhibited desired encapsulation efficiencies of ∼91.2 and 71.9% for CIS and EPI, respectively. Moreover, cellular assays disclosed that a CIS and EPI-loaded niosome (NCE) and FA-PEGylated niosomal CIS and EPI (FPNCE) enhanced the apoptosis rate and cell migration in SKBR3 and 4T1 cells compared to CIS, EPI, and their combination (CIS+EPI). For FPNCE and NCE groups, the expression levels of Bax , Caspase3 , Caspase9 , and Mfn1 genes increased, whereas the expression of Bcl2 , Drp1 , MMP-2 , and MMP-9 genes was downregulated. Histopathology results showed a reduction in the mitosis index, invasion, and pleomorphism in BALB/c inbred mice with NCE and FPNCE treatment. In this paper, for the first time, we report a niosomal nanocarrier functionalized with PEG and FA for codelivery of CIS and EPI to treat breast cancer. The results demonstrated that the codelivery of CIS and EPI through FA-PEGylated niosomes holds great potential for breast cancer treatment.