Open AccessEngineered Biosynthesis of Fungal 4-Quinolone Natural Products
Author(s) -
Mengting Liu,
M. Ohashi,
Yi Tang
Publication year - 2020
Publication title -
organic letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.94
H-Index - 239
eISSN - 1523-7060
pISSN - 1523-7052
DOI - 10.1021/acs.orglett.0c02426
Subject(s) - quinolone , heterologous , nonribosomal peptide , chemistry , biosynthesis , heterologous expression , penicillium , enzyme , bacteria , in vitro , biochemistry , microbiology and biotechnology , stereochemistry , biology , antibiotics , recombinant dna , gene , genetics , food science
Quinolone-containing natural products are widely found in bacteria, fungi, and plants. The fungal quinolactacins, which are N- methyl-4-quinolones, display a wide spectrum of biological activities. Here we uncovered a concise nonribosomal peptide synthetase pathway involved in quinolactacin A biosynthesis from Penicillium by using heterologous reconstitution and in vitro enzymatic synthesis. The N- desmethyl analog of quinolactacin A was accessed through the construction of a hybrid bacterial and fungi pathway in the heterologous host.