Whole-Cell, 3D, and Multicolor STED Imaging with Exchangeable Fluorophores | Zendy

Christoph Spahn | Zendy; Jonathan B. Grimm | Zendy; Luke D. Lavis | Zendy; Marko Lampe | Zendy; Mike Heilemann | Zendy

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Whole-Cell, 3D, and Multicolor STED Imaging with Exchangeable Fluorophores

Author(s) -

Christoph Spahn,

Jonathan B. Grimm,

Luke D. Lavis,

Marko Lampe,

Mike Heilemann

Publication year - 2018

Publication title -

nano letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 4.853

H-Index - 488

eISSN - 1530-6992

pISSN - 1530-6984

DOI - 10.1021/acs.nanolett.8b04385

Subject(s) - sted microscopy , fluorescence , microscopy , fluorescence microscope , chemistry , stimulated emission , resolution (logic) , superresolution , biophysics , nanotechnology , optics , laser , materials science , physics , biology , computer science , artificial intelligence , image (mathematics)

We demonstrate stimulated emission depletion (STED) microscopy of whole bacterial and eukaryotic cells using fluorogenic labels that reversibly bind to their target structure. A constant exchange of labels guarantees the removal of photobleached fluorophores and their replacement by intact fluorophores, thereby circumventing bleaching-related limitations of STED super-resolution imaging. We achieve a constant labeling density and demonstrate a fluorescence signal for long and theoretically unlimited acquisition times. Using this concept, we demonstrate whole-cell, 3D, multicolor, and live-cell STED microscopy.

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