Open AccessNanoscale Control of Surface Immobilized BMP-2: Toward a Quantitative Assessment of BMP-Mediated Signaling Events
Author(s) -
Elisabeth H. Schwab,
Theresa L. M. Pohl,
Tamás Haraszti,
Gerburg K. Schwaerzer,
Christian Hiepen,
Joachim P. Spatz,
Petra Knaus,
Elisabetta Ada CavalcantiAdam
Publication year - 2015
Publication title -
nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.853
H-Index - 488
eISSN - 1530-6992
pISSN - 1530-6984
DOI - 10.1021/acs.nanolett.5b00315
Subject(s) - nanolithography , smad , nanotechnology , nanotopography , nanoscopic scale , signal transduction , bone morphogenetic protein 2 , intracellular , materials science , biophysics , chemistry , biochemistry , in vitro , biology , medicine , alternative medicine , pathology , fabrication
In this work we determine the impact of surface density of immobilized BMP-2 on intracellular signal transduction. We use block copolymer micellar nanolithography to fabricate substrates with precisely spaced and tunable gold nanoparticle arrays carrying single BMP-2 molecules. We found that the immobilized growth factor triggers prolonged and elevated Smad signaling pathway activation compared to the same amount of soluble protein. This approach is suitable for achieving controlled and sustained local delivery of BMP-2 and other growth factors.