Open AccessMorphologic Design of Silver-Bearing Sugar-Based Polymer Nanoparticles for Uroepithelial Cell Binding and Antimicrobial Delivery
Author(s) -
Yue Song,
Mahmoud Elsabahy,
Christina A. Collins,
Sarosh Khan,
Richen Li,
Teri N. Hreha,
Yidan Shen,
YenNan Lin,
Rachel Letteri,
Lu Su,
Mei Dong,
Fuwu Zhang,
David A. Hunstad,
Karen L. Wooley
Publication year - 2021
Publication title -
nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.853
H-Index - 488
eISSN - 1530-6992
pISSN - 1530-6984
DOI - 10.1021/acs.nanolett.1c00776
Subject(s) - polymer , antimicrobial , materials science , nanoparticle , biophysics , flow cytometry , cytotoxicity , internalization , crystallinity , nanostructure , nanotechnology , in vitro , chemistry , cell , chemical engineering , microbiology and biotechnology , organic chemistry , biochemistry , biology , immunology , composite material , engineering
Platelet-like and cylindrical nanostructures from sugar-based polymers are designed to mimic the aspect ratio of bacteria and achieve uroepithelial cell binding and internalization, thereby improving their potential for local treatment of recurrent urinary tract infections. Polymer nanostructures, derived from amphiphilic block polymers composed of zwitterionic poly(d-glucose carbonate) and semicrystalline poly(l-lactide) segments, were constructed with morphologies that could be tuned to enhance uroepithelial cell binding. These nanoparticles exhibited negligible cytotoxicity, immunotoxicity, and cytokine adsorption, while also offering substantial silver cation loading capacity, extended release, and in vitro antimicrobial activity (as effective as free silver cations) against uropathogenic Escherichia coli . In comparison to spherical analogues, cylindrical and platelet-like nanostructures engaged in significantly higher association with uroepithelial cells, as measured by flow cytometry; despite their larger size, platelet-like nanostructures maintained the capacity for cell internalization. This work establishes initial evidence of degradable platelet-shaped nanostructures as versatile therapeutic carriers for treatment of epithelial infections.