Open AccessImaging of Cell Morphology Changes via Metamaterial-Assisted Photobleaching Microscopy
Author(s) -
Yeon Ui Lee,
Clara Posner,
Junxiao Zhou,
Jin Zhang,
Zhaowei Liu
Publication year - 2021
Publication title -
nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.853
H-Index - 488
eISSN - 1530-6992
pISSN - 1530-6984
DOI - 10.1021/acs.nanolett.0c04529
Subject(s) - photobleaching , microscopy , resolution (logic) , materials science , live cell imaging , morphology (biology) , metamaterial , nanoscopic scale , optics , nanotechnology , chemistry , optoelectronics , physics , cell , biology , fluorescence , biochemistry , genetics , artificial intelligence , computer science
Determining the axial position of an emitter with nanoscale precision is critical to a fundamental imaging methodology. While there are many advanced optical techniques being applied to high-resolution imaging, high-axial-resolution topography imaging of living cells is particularly challenging. Here, we present an application of metamaterial-assisted photobleaching microscopy (MAPM) with high-axial resolution to characterize morphological properties of living cells. Quantitative imaging of changes in the morphology of live cells is obtained by topographic and statistical analysis. The time-lapse topography image using the metamaterial-induced photostability provides information about growth factor induced changes in the cell morphology with high-axial resolution.