Open AccessLongitudinal Transcriptomic, Proteomic, and Metabolomic Analysis of Citrus limon Response to Graft Inoculation by Candidatus Liberibacter asiaticus
Author(s) -
John S. Ramsey,
Elizabeth Chin,
Juan D. Chavez,
Surya Saha,
Darya Mischuk,
Jaclyn Mahoney,
Jared P. Mohr,
F. Robison,
Elizabeth Mitrovic,
Youqiang Xu,
Susan R. Strickler,
Noe Fernandez,
Xuefei Zhong,
MaryLou Polek,
Kris Godfrey,
James J. Giovani,
Lukas A. Mueller,
Carolyn M. Slupsky,
James E. Bruce,
Michelle Heck
Publication year - 2020
Publication title -
journal of proteome research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.644
H-Index - 161
eISSN - 1535-3907
pISSN - 1535-3893
DOI - 10.1021/acs.jproteome.9b00802
Subject(s) - biology , transcriptome , metabolomics , inoculation , proteome , diaphorina citri , pathogen , horticulture , botany , microbiology and biotechnology , gene , gene expression , genetics , bioinformatics , hemiptera
Presymptomatic detection of citrus trees infected with Candidatus Liberibacter asiaticus ( C Las), the bacterial pathogen associated with Huanglongbing (HLB; citrus greening disease), is critical to controlling the spread of the disease. To test whether infected citrus trees produce systemic signals that may be used for indirect disease detection, lemon ( Citrus limon ) plants were graft-inoculated with either C Las-infected or control ( C Las-) budwood, and leaf samples were longitudinally collected over 46 weeks and analyzed for plant changes associated with C Las infection. RNA, protein, and metabolite samples extracted from leaves were analyzed using RNA-Seq, mass spectrometry, and 1 H NMR spectroscopy, respectively. Significant differences in specific transcripts, proteins, and metabolites were observed between C Las-infected and control plants as early as 2 weeks post graft (wpg). The most dramatic differences between the transcriptome and proteome of C Las-infected and control plants were observed at 10 wpg, including coordinated increases in transcripts and proteins of citrus orthologs of known plant defense genes. This integrated approach to quantifying plant molecular changes in leaves of C Las-infected plants supports the development of diagnostic technology for presymptomatic or early disease detection as part of efforts to control the spread of HLB into uninfected citrus groves.